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FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1971..2067) /label=attL2 /note="attL2 (c)" misc_signal complement(2082..2101) /note="T7 Promoter/priming site (c)" primer_bind 2109..2125 /note="M13 Reverse priming site" gene 2238..3047 /note="Kanamycin resistance gene" rep_origin 3168..3841 /note="pUC origin" vector join(1979..3844,1..651) /source="pDONR%99221" /type="Donor Vector" CDS 678..1967 /label=ORF1 misc_feature 672..677 /note="Kozak seq" misc_feature 684..707 /note="His Tag" misc_feature 714..737 /note="TEV protease site" misc_feature 738..1969 /note="ISPD coding region" source 1..3844 /dnas_title="ISPD-pDONR" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CGCCACCATG GCCCACCACC ATCATCACCA TCACCACGAG TTCGAGAACC 721 TGTACTTCCA GAGCGGCATG CACCCTCAGG CCGTGGCTGC TGTGCTGCCT GCTGGCGGAT 781 GTGGCGAGAG AATGGGCGTG CCAACCCCCA AGCAGTTCTG CCCCATCCTG GAACGGCCCC 841 TGATCAGCTA CACACTGCAG GCCCTGGAAA GAGTGTGCTG GATCAAGGAC ATCGTGGTGG 901 CCGTGACCGG CGAGAACATG GAAGTGATGA AGTCCATCAT CCAGAAGTAC CAGCACAAGC 961 GGATCAGCCT GGTGGAAGCC GGCGTGACCA GACACCGGTC CATCTTCAAT GGCCTGAAGG 1021 CCCTGGCCGA GGACCAGATC AACAGCAAGC TGAGCAAGCC CGAGGTCGTG ATCATCCACG 1081 ATGCCGTGCG GCCCTTCGTG GAAGAAGGCG TGCTGCTGAA GGTCGTGACC GCCGCCAAAG 1141 AACATGGCGC CGCTGGCGCT ATCAGACCCC TGGTGTCTAC CGTGGTGTCC CCTAGCGCCG 1201 ATGGCTGCCT GGACTACAGC CTGGAAAGGG CCAGACACAG AGCCAGCGAG ATGCCCCAGG 1261 CCTTCCTGTT CGACGTGATC TACGAGGCCT ACCAGCAGTG CAGCGACTAC GACCTGGAAT 1321 TCGGCACCGA GTGCCTGCAG CTGGCCCTGA AGTACTGCTG CACCAAGGCC AAGCTGGTGG 1381 AAGGCAGCCC CGACCTGTGG AAAGTGACCT ACAAGCGGGA CCTGTACGCC GCCGAGAGCA 1441 TCATCAAAGA GAGAATCAGC CAGGAAATCT GTGTCGTGAT GGACACCGAA GAGGACAACA 1501 AGCACGTGGG CCATCTGCTG GAAGAGGTGC TGAAGTCCGA GCTGAACCAC GTGAAAGTGA 1561 CAAGCGAGGC CCTGGGCCAC GCCGGCAGAC ATCTGCAGCA GATCATCCTG GACCAGTGCT 1621 ACAACTTCGT GTGCGTGAAC GTGACCACCA GCGACTTCCA GGAAACCCAG AAACTGCTGA 1681 GCATGCTGGA AGAAAGCAGC CTGTGCATCC TGTACCCCGT GGTGGTGGTG TCCGTGCACT 1741 TCCTGGACTT TAAGCTGGTG CCCCCCAGCC AGAAAATGGA AAACCTGATG CAGATCCGCG 1801 AGTTTGCCAA AGAAGTGAAA GAGCGGAACA TCCTGCTGTA CGGACTGCTG ATTAGCTACC 1861 CCCAGGACGA CCAGAAGCTG CAGGAAAGCC TGAGACAGGG CGCCATCATC ATTGCCAGCC 1921 TGATCAAAGA ACGGAACAGC GGCCTGATCG GACAGCTGCT GATTGCCTGA CCAGCTTTCT 1981 TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA 2041 CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT 2101 ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT 2161 ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA 2221 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT 2281 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG 2341 GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG 2401 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG 2461 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC 2521 TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG 2581 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT 2641 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA 2701 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC 2761 AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG 2821 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG 2881 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG 2941 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG 3001 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA 3061 ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC 3121 CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 3181 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 3241 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 3301 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT 3361 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 3421 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 3481 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 3541 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA 3601 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 3661 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 3721 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 3781 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 3841 TGTT //